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MetaMorph Inc metamorph software
Ablating Pcbp1 in β cells upregulates PCBP2 and does not alter glucose homeostasis. (A) Model of Cre-mediated recombination of Pcbp1 . The top diagram represents the Pcbp1 locus (light boxes represent UTRs, dark box represents protein coding region, and arrow represents transcriptional start site) with inserted loxP sites (triangles). Bottom diagram represents the Pcbp1 locus following RIP-Cre mediated recombination and remaining loxP site (triangle). (B) Western blot showing PCBP1 levels in islets from 26 week old male Pcbp1 βKO mice ( Pcbp1 Fl/Fl ,n = 3; Pcbp1 βKO ,n = 3). (C) <t>Co-immunofluorescence</t> staining for PCBP1 and Insulin in adult islets from 7 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice (scale bar, 50 μm). (D) Intraperitoneal glucose tolerance tests performed on 8–11 week old male mice (n = 9 Pcbp1 Fl/Fl and n = 11 Pcbp1 βKO ). (E) Western blots showing PCBP2 levels in islets from 26 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice ( Pcbp1 Fl/Fl , n = 3; Pcbp1 βKO , n = 3). ∗P-value<0.05, ∗∗P-value<0.01 by student's t-test.
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1) Product Images from "RNA binding proteins PCBP1 and PCBP2 regulate pancreatic β cell translation"

Article Title: RNA binding proteins PCBP1 and PCBP2 regulate pancreatic β cell translation

Journal: Molecular Metabolism

doi: 10.1016/j.molmet.2025.102175

Ablating Pcbp1 in β cells upregulates PCBP2 and does not alter glucose homeostasis. (A) Model of Cre-mediated recombination of Pcbp1 . The top diagram represents the Pcbp1 locus (light boxes represent UTRs, dark box represents protein coding region, and arrow represents transcriptional start site) with inserted loxP sites (triangles). Bottom diagram represents the Pcbp1 locus following RIP-Cre mediated recombination and remaining loxP site (triangle). (B) Western blot showing PCBP1 levels in islets from 26 week old male Pcbp1 βKO mice ( Pcbp1 Fl/Fl ,n = 3; Pcbp1 βKO ,n = 3). (C) Co-immunofluorescence staining for PCBP1 and Insulin in adult islets from 7 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice (scale bar, 50 μm). (D) Intraperitoneal glucose tolerance tests performed on 8–11 week old male mice (n = 9 Pcbp1 Fl/Fl and n = 11 Pcbp1 βKO ). (E) Western blots showing PCBP2 levels in islets from 26 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice ( Pcbp1 Fl/Fl , n = 3; Pcbp1 βKO , n = 3). ∗P-value<0.05, ∗∗P-value<0.01 by student's t-test.
Figure Legend Snippet: Ablating Pcbp1 in β cells upregulates PCBP2 and does not alter glucose homeostasis. (A) Model of Cre-mediated recombination of Pcbp1 . The top diagram represents the Pcbp1 locus (light boxes represent UTRs, dark box represents protein coding region, and arrow represents transcriptional start site) with inserted loxP sites (triangles). Bottom diagram represents the Pcbp1 locus following RIP-Cre mediated recombination and remaining loxP site (triangle). (B) Western blot showing PCBP1 levels in islets from 26 week old male Pcbp1 βKO mice ( Pcbp1 Fl/Fl ,n = 3; Pcbp1 βKO ,n = 3). (C) Co-immunofluorescence staining for PCBP1 and Insulin in adult islets from 7 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice (scale bar, 50 μm). (D) Intraperitoneal glucose tolerance tests performed on 8–11 week old male mice (n = 9 Pcbp1 Fl/Fl and n = 11 Pcbp1 βKO ). (E) Western blots showing PCBP2 levels in islets from 26 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice ( Pcbp1 Fl/Fl , n = 3; Pcbp1 βKO , n = 3). ∗P-value<0.05, ∗∗P-value<0.01 by student's t-test.

Techniques Used: Western Blot, Immunofluorescence, Staining

Single cell RNA sequencing of Pcbp1 and Pcbp2 co-depleted β cells reveals dysregulation of a translational mRNA program. (A) Co-immunofluorescence staining for PCBP1 and PCBP2 in adult islets from 11–12 week old male Pcbp1/2 Fl/Fl and Pcbp1/2 βKO mice (scale bar, 50 μm). Yellow arrows denote PCBP1 and PCBP2 co-depleted β cells. (B) Merged uniform manifold approximation and projection (UMAP) plot showing 3,592 control β cells (red) and 1,554 Pcbp1/2 co-depleted β cells (blue). (C) UMAP plot showing identified β cell subclusters in control and mutant β cells. (D) Dotplot showing the relative expression of Ins1 , Pcbp1 , and Pcbp2 transcript levels in each identified β cell subcluster. (E) Volcano plot of genes with significantly downregulated (blue) and upregulated (red) transcript levels in cluster 11 as compared to control β cells. (F) Reactome pathway analysis of the genes with altered mRNA levels in the setting of Pcbp1/2 co-deficiency.
Figure Legend Snippet: Single cell RNA sequencing of Pcbp1 and Pcbp2 co-depleted β cells reveals dysregulation of a translational mRNA program. (A) Co-immunofluorescence staining for PCBP1 and PCBP2 in adult islets from 11–12 week old male Pcbp1/2 Fl/Fl and Pcbp1/2 βKO mice (scale bar, 50 μm). Yellow arrows denote PCBP1 and PCBP2 co-depleted β cells. (B) Merged uniform manifold approximation and projection (UMAP) plot showing 3,592 control β cells (red) and 1,554 Pcbp1/2 co-depleted β cells (blue). (C) UMAP plot showing identified β cell subclusters in control and mutant β cells. (D) Dotplot showing the relative expression of Ins1 , Pcbp1 , and Pcbp2 transcript levels in each identified β cell subcluster. (E) Volcano plot of genes with significantly downregulated (blue) and upregulated (red) transcript levels in cluster 11 as compared to control β cells. (F) Reactome pathway analysis of the genes with altered mRNA levels in the setting of Pcbp1/2 co-deficiency.

Techniques Used: RNA Sequencing, Immunofluorescence, Staining, Control, Mutagenesis, Expressing

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Immunofluorescence:

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Article Snippet: Metamorph® Software was used to quantify Tryptase+ and TLR7+ cells.

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Immunostaining:

Article Title: Evidence of inter- and intra-keloid heterogeneity through analysis of dermal fibroblasts: A new insight in deciphering keloid physiopathology.
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Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase
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Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase.
Article Snippet: Metamorph® Software was used to quantify Tryptase+ and TLR7+ cells.

Article Title: Heteromultivalent particle compositions
Article Snippet: Data Analysis For in vitro and ex vivo fluorescence images of liposome-incubated platelets, surface averaged fluorescence intensity was measured using Metamorph® software.

Article Title: NLRP12 is an innate immune checkpoint for repressing IFN signatures and attenuating lupus nephritis progression
Article Snippet: Flowchart of calculating PAS staining area of kidney section by metamorph® software.

Control:

Article Title: Evidence of inter- and intra-keloid heterogeneity through analysis of dermal fibroblasts: A new insight in deciphering keloid physiopathology.
Article Snippet: © 2023 John Wiley & Sons A/S.. Published by John Wiley & Sons Ltd. Kévin Serror and Lauren Ferrero cofirst authors who equally contributed to this work.. 1INSERM UMR_S 976, Skin Research Center, SaintLouis Hospital, Paris, France 2Paris University, Paris, France 3Department of Reconstructive and Plastic Surgery, SaintLouis Hospital, Paris, France 4INSERM UMR_S 976, CEA CytoMorphoLab, SaintLouis Hospital, Paris, France 5Genomics Platform, Translational Research Department, Research Center, Institut Curie, Paris Sciences et Lettres (PSL) Research University, Paris, France 6GenoSplice, Paris Biotech Santé, Paris, France 7Dermatology Department, SaintLouis Hospital, Paris, France

Article Title: Reply to: High-Dose Inhaled Nitric Oxide in Acute Hypoxemic Respiratory Failure: Need for Patient Phenotyping?
Article Snippet: Rationale: Within chronic obstructive pulmonary disease (COPD), emphysema is characterized by a significant yet partially understood B cell immune component.. Objective: To characterize the transcriptomic signatures from lymphoid follicles (LFs) in ever-smokers without COPD and COPD patients with varying degrees of emphysema.. Methods: Lung sections from 40 COPD patients and ever-smokers were used for LF proteomic and transcriptomic spatial profiling.

Article Title: Duox is the primary NADPH oxidase responsible for ROS production during adult caudal fin regeneration in zebrafish
Article Snippet: MetaMorph® software was used to setup the mircroscope to capture two wavelengths - HyPer low (CFP) emission 482/25 and HyPer high (YFP) emission 544/24.

Article Title: Antibodies and antigen-binding fragments that specifically bind to microtubule-associated protein tau
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Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase
Article Snippet: Metamorph® Software was used to quantify Tryptase + and TLR7 + cells.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase.
Article Snippet: Metamorph® Software was used to quantify Tryptase+ and TLR7+ cells.

Article Title: Heteromultivalent particle compositions
Article Snippet: Data Analysis For in vitro and ex vivo fluorescence images of liposome-incubated platelets, surface averaged fluorescence intensity was measured using Metamorph® software.

Article Title: NLRP12 is an innate immune checkpoint for repressing IFN signatures and attenuating lupus nephritis progression
Article Snippet: Flowchart of calculating PAS staining area of kidney section by metamorph® software.

Comparison:

Article Title: Evidence of inter- and intra-keloid heterogeneity through analysis of dermal fibroblasts: A new insight in deciphering keloid physiopathology.
Article Snippet: © 2023 John Wiley & Sons A/S.. Published by John Wiley & Sons Ltd. Kévin Serror and Lauren Ferrero cofirst authors who equally contributed to this work.. 1INSERM UMR_S 976, Skin Research Center, SaintLouis Hospital, Paris, France 2Paris University, Paris, France 3Department of Reconstructive and Plastic Surgery, SaintLouis Hospital, Paris, France 4INSERM UMR_S 976, CEA CytoMorphoLab, SaintLouis Hospital, Paris, France 5Genomics Platform, Translational Research Department, Research Center, Institut Curie, Paris Sciences et Lettres (PSL) Research University, Paris, France 6GenoSplice, Paris Biotech Santé, Paris, France 7Dermatology Department, SaintLouis Hospital, Paris, France

Article Title: Reply to: High-Dose Inhaled Nitric Oxide in Acute Hypoxemic Respiratory Failure: Need for Patient Phenotyping?
Article Snippet: Rationale: Within chronic obstructive pulmonary disease (COPD), emphysema is characterized by a significant yet partially understood B cell immune component.. Objective: To characterize the transcriptomic signatures from lymphoid follicles (LFs) in ever-smokers without COPD and COPD patients with varying degrees of emphysema.. Methods: Lung sections from 40 COPD patients and ever-smokers were used for LF proteomic and transcriptomic spatial profiling.

Article Title: Duox is the primary NADPH oxidase responsible for ROS production during adult caudal fin regeneration in zebrafish
Article Snippet: MetaMorph® software was used to setup the mircroscope to capture two wavelengths - HyPer low (CFP) emission 482/25 and HyPer high (YFP) emission 544/24.

Article Title: Antibodies and antigen-binding fragments that specifically bind to microtubule-associated protein tau
Article Snippet: Representative images were acquired with Olympus BX-41 upright microscope using METAMORPH® software.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase
Article Snippet: Metamorph® Software was used to quantify Tryptase + and TLR7 + cells.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase.
Article Snippet: Metamorph® Software was used to quantify Tryptase+ and TLR7+ cells.

Article Title: Heteromultivalent particle compositions
Article Snippet: Data Analysis For in vitro and ex vivo fluorescence images of liposome-incubated platelets, surface averaged fluorescence intensity was measured using Metamorph® software.

Article Title: NLRP12 is an innate immune checkpoint for repressing IFN signatures and attenuating lupus nephritis progression
Article Snippet: Flowchart of calculating PAS staining area of kidney section by metamorph® software.

Two Tailed Test:

Article Title: Evidence of inter- and intra-keloid heterogeneity through analysis of dermal fibroblasts: A new insight in deciphering keloid physiopathology.
Article Snippet: © 2023 John Wiley & Sons A/S.. Published by John Wiley & Sons Ltd. Kévin Serror and Lauren Ferrero cofirst authors who equally contributed to this work.. 1INSERM UMR_S 976, Skin Research Center, SaintLouis Hospital, Paris, France 2Paris University, Paris, France 3Department of Reconstructive and Plastic Surgery, SaintLouis Hospital, Paris, France 4INSERM UMR_S 976, CEA CytoMorphoLab, SaintLouis Hospital, Paris, France 5Genomics Platform, Translational Research Department, Research Center, Institut Curie, Paris Sciences et Lettres (PSL) Research University, Paris, France 6GenoSplice, Paris Biotech Santé, Paris, France 7Dermatology Department, SaintLouis Hospital, Paris, France

Article Title: Reply to: High-Dose Inhaled Nitric Oxide in Acute Hypoxemic Respiratory Failure: Need for Patient Phenotyping?
Article Snippet: Rationale: Within chronic obstructive pulmonary disease (COPD), emphysema is characterized by a significant yet partially understood B cell immune component.. Objective: To characterize the transcriptomic signatures from lymphoid follicles (LFs) in ever-smokers without COPD and COPD patients with varying degrees of emphysema.. Methods: Lung sections from 40 COPD patients and ever-smokers were used for LF proteomic and transcriptomic spatial profiling.

Article Title: Duox is the primary NADPH oxidase responsible for ROS production during adult caudal fin regeneration in zebrafish
Article Snippet: MetaMorph® software was used to setup the mircroscope to capture two wavelengths - HyPer low (CFP) emission 482/25 and HyPer high (YFP) emission 544/24.

Article Title: Antibodies and antigen-binding fragments that specifically bind to microtubule-associated protein tau
Article Snippet: Representative images were acquired with Olympus BX-41 upright microscope using METAMORPH® software.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase
Article Snippet: Metamorph® Software was used to quantify Tryptase + and TLR7 + cells.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase.
Article Snippet: Metamorph® Software was used to quantify Tryptase+ and TLR7+ cells.

Article Title: Heteromultivalent particle compositions
Article Snippet: Data Analysis For in vitro and ex vivo fluorescence images of liposome-incubated platelets, surface averaged fluorescence intensity was measured using Metamorph® software.

Article Title: NLRP12 is an innate immune checkpoint for repressing IFN signatures and attenuating lupus nephritis progression
Article Snippet: Flowchart of calculating PAS staining area of kidney section by metamorph® software.

MANN-WHITNEY:

Article Title: Evidence of inter- and intra-keloid heterogeneity through analysis of dermal fibroblasts: A new insight in deciphering keloid physiopathology.
Article Snippet: © 2023 John Wiley & Sons A/S.. Published by John Wiley & Sons Ltd. Kévin Serror and Lauren Ferrero cofirst authors who equally contributed to this work.. 1INSERM UMR_S 976, Skin Research Center, SaintLouis Hospital, Paris, France 2Paris University, Paris, France 3Department of Reconstructive and Plastic Surgery, SaintLouis Hospital, Paris, France 4INSERM UMR_S 976, CEA CytoMorphoLab, SaintLouis Hospital, Paris, France 5Genomics Platform, Translational Research Department, Research Center, Institut Curie, Paris Sciences et Lettres (PSL) Research University, Paris, France 6GenoSplice, Paris Biotech Santé, Paris, France 7Dermatology Department, SaintLouis Hospital, Paris, France

Article Title: Reply to: High-Dose Inhaled Nitric Oxide in Acute Hypoxemic Respiratory Failure: Need for Patient Phenotyping?
Article Snippet: Rationale: Within chronic obstructive pulmonary disease (COPD), emphysema is characterized by a significant yet partially understood B cell immune component.. Objective: To characterize the transcriptomic signatures from lymphoid follicles (LFs) in ever-smokers without COPD and COPD patients with varying degrees of emphysema.. Methods: Lung sections from 40 COPD patients and ever-smokers were used for LF proteomic and transcriptomic spatial profiling.

Article Title: Duox is the primary NADPH oxidase responsible for ROS production during adult caudal fin regeneration in zebrafish
Article Snippet: MetaMorph® software was used to setup the mircroscope to capture two wavelengths - HyPer low (CFP) emission 482/25 and HyPer high (YFP) emission 544/24.

Article Title: Antibodies and antigen-binding fragments that specifically bind to microtubule-associated protein tau
Article Snippet: Representative images were acquired with Olympus BX-41 upright microscope using METAMORPH® software.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase
Article Snippet: Metamorph® Software was used to quantify Tryptase + and TLR7 + cells.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase.
Article Snippet: Metamorph® Software was used to quantify Tryptase+ and TLR7+ cells.

Article Title: Heteromultivalent particle compositions
Article Snippet: Data Analysis For in vitro and ex vivo fluorescence images of liposome-incubated platelets, surface averaged fluorescence intensity was measured using Metamorph® software.

Article Title: NLRP12 is an innate immune checkpoint for repressing IFN signatures and attenuating lupus nephritis progression
Article Snippet: Flowchart of calculating PAS staining area of kidney section by metamorph® software.

TUNEL Assay:

Article Title: Evidence of inter- and intra-keloid heterogeneity through analysis of dermal fibroblasts: A new insight in deciphering keloid physiopathology.
Article Snippet: © 2023 John Wiley & Sons A/S.. Published by John Wiley & Sons Ltd. Kévin Serror and Lauren Ferrero cofirst authors who equally contributed to this work.. 1INSERM UMR_S 976, Skin Research Center, SaintLouis Hospital, Paris, France 2Paris University, Paris, France 3Department of Reconstructive and Plastic Surgery, SaintLouis Hospital, Paris, France 4INSERM UMR_S 976, CEA CytoMorphoLab, SaintLouis Hospital, Paris, France 5Genomics Platform, Translational Research Department, Research Center, Institut Curie, Paris Sciences et Lettres (PSL) Research University, Paris, France 6GenoSplice, Paris Biotech Santé, Paris, France 7Dermatology Department, SaintLouis Hospital, Paris, France

Article Title: Reply to: High-Dose Inhaled Nitric Oxide in Acute Hypoxemic Respiratory Failure: Need for Patient Phenotyping?
Article Snippet: Rationale: Within chronic obstructive pulmonary disease (COPD), emphysema is characterized by a significant yet partially understood B cell immune component.. Objective: To characterize the transcriptomic signatures from lymphoid follicles (LFs) in ever-smokers without COPD and COPD patients with varying degrees of emphysema.. Methods: Lung sections from 40 COPD patients and ever-smokers were used for LF proteomic and transcriptomic spatial profiling.

Article Title: Duox is the primary NADPH oxidase responsible for ROS production during adult caudal fin regeneration in zebrafish
Article Snippet: MetaMorph® software was used to setup the mircroscope to capture two wavelengths - HyPer low (CFP) emission 482/25 and HyPer high (YFP) emission 544/24.

Article Title: Antibodies and antigen-binding fragments that specifically bind to microtubule-associated protein tau
Article Snippet: Representative images were acquired with Olympus BX-41 upright microscope using METAMORPH® software.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase
Article Snippet: Metamorph® Software was used to quantify Tryptase + and TLR7 + cells.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase.
Article Snippet: Metamorph® Software was used to quantify Tryptase+ and TLR7+ cells.

Article Title: Heteromultivalent particle compositions
Article Snippet: Data Analysis For in vitro and ex vivo fluorescence images of liposome-incubated platelets, surface averaged fluorescence intensity was measured using Metamorph® software.

Article Title: NLRP12 is an innate immune checkpoint for repressing IFN signatures and attenuating lupus nephritis progression
Article Snippet: Flowchart of calculating PAS staining area of kidney section by metamorph® software.

Membrane:

Article Title: Evidence of inter- and intra-keloid heterogeneity through analysis of dermal fibroblasts: A new insight in deciphering keloid physiopathology.
Article Snippet: © 2023 John Wiley & Sons A/S.. Published by John Wiley & Sons Ltd. Kévin Serror and Lauren Ferrero cofirst authors who equally contributed to this work.. 1INSERM UMR_S 976, Skin Research Center, SaintLouis Hospital, Paris, France 2Paris University, Paris, France 3Department of Reconstructive and Plastic Surgery, SaintLouis Hospital, Paris, France 4INSERM UMR_S 976, CEA CytoMorphoLab, SaintLouis Hospital, Paris, France 5Genomics Platform, Translational Research Department, Research Center, Institut Curie, Paris Sciences et Lettres (PSL) Research University, Paris, France 6GenoSplice, Paris Biotech Santé, Paris, France 7Dermatology Department, SaintLouis Hospital, Paris, France

Article Title: Reply to: High-Dose Inhaled Nitric Oxide in Acute Hypoxemic Respiratory Failure: Need for Patient Phenotyping?
Article Snippet: Rationale: Within chronic obstructive pulmonary disease (COPD), emphysema is characterized by a significant yet partially understood B cell immune component.. Objective: To characterize the transcriptomic signatures from lymphoid follicles (LFs) in ever-smokers without COPD and COPD patients with varying degrees of emphysema.. Methods: Lung sections from 40 COPD patients and ever-smokers were used for LF proteomic and transcriptomic spatial profiling.

Article Title: Duox is the primary NADPH oxidase responsible for ROS production during adult caudal fin regeneration in zebrafish
Article Snippet: MetaMorph® software was used to setup the mircroscope to capture two wavelengths - HyPer low (CFP) emission 482/25 and HyPer high (YFP) emission 544/24.

Article Title: Antibodies and antigen-binding fragments that specifically bind to microtubule-associated protein tau
Article Snippet: Representative images were acquired with Olympus BX-41 upright microscope using METAMORPH® software.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase
Article Snippet: Metamorph® Software was used to quantify Tryptase + and TLR7 + cells.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase.
Article Snippet: Metamorph® Software was used to quantify Tryptase+ and TLR7+ cells.

Article Title: Heteromultivalent particle compositions
Article Snippet: Data Analysis For in vitro and ex vivo fluorescence images of liposome-incubated platelets, surface averaged fluorescence intensity was measured using Metamorph® software.

Article Title: NLRP12 is an innate immune checkpoint for repressing IFN signatures and attenuating lupus nephritis progression
Article Snippet: Flowchart of calculating PAS staining area of kidney section by metamorph® software.

Immunohistochemistry:

Article Title: Evidence of inter- and intra-keloid heterogeneity through analysis of dermal fibroblasts: A new insight in deciphering keloid physiopathology.
Article Snippet: © 2023 John Wiley & Sons A/S.. Published by John Wiley & Sons Ltd. Kévin Serror and Lauren Ferrero cofirst authors who equally contributed to this work.. 1INSERM UMR_S 976, Skin Research Center, SaintLouis Hospital, Paris, France 2Paris University, Paris, France 3Department of Reconstructive and Plastic Surgery, SaintLouis Hospital, Paris, France 4INSERM UMR_S 976, CEA CytoMorphoLab, SaintLouis Hospital, Paris, France 5Genomics Platform, Translational Research Department, Research Center, Institut Curie, Paris Sciences et Lettres (PSL) Research University, Paris, France 6GenoSplice, Paris Biotech Santé, Paris, France 7Dermatology Department, SaintLouis Hospital, Paris, France

Article Title: Reply to: High-Dose Inhaled Nitric Oxide in Acute Hypoxemic Respiratory Failure: Need for Patient Phenotyping?
Article Snippet: Rationale: Within chronic obstructive pulmonary disease (COPD), emphysema is characterized by a significant yet partially understood B cell immune component.. Objective: To characterize the transcriptomic signatures from lymphoid follicles (LFs) in ever-smokers without COPD and COPD patients with varying degrees of emphysema.. Methods: Lung sections from 40 COPD patients and ever-smokers were used for LF proteomic and transcriptomic spatial profiling.

Article Title: Duox is the primary NADPH oxidase responsible for ROS production during adult caudal fin regeneration in zebrafish
Article Snippet: MetaMorph® software was used to setup the mircroscope to capture two wavelengths - HyPer low (CFP) emission 482/25 and HyPer high (YFP) emission 544/24.

Article Title: Antibodies and antigen-binding fragments that specifically bind to microtubule-associated protein tau
Article Snippet: Representative images were acquired with Olympus BX-41 upright microscope using METAMORPH® software.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase
Article Snippet: Metamorph® Software was used to quantify Tryptase + and TLR7 + cells.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase.
Article Snippet: Metamorph® Software was used to quantify Tryptase+ and TLR7+ cells.

Article Title: Heteromultivalent particle compositions
Article Snippet: Data Analysis For in vitro and ex vivo fluorescence images of liposome-incubated platelets, surface averaged fluorescence intensity was measured using Metamorph® software.

Article Title: NLRP12 is an innate immune checkpoint for repressing IFN signatures and attenuating lupus nephritis progression
Article Snippet: Flowchart of calculating PAS staining area of kidney section by metamorph® software.

Sterility:

Article Title: Evidence of inter- and intra-keloid heterogeneity through analysis of dermal fibroblasts: A new insight in deciphering keloid physiopathology.
Article Snippet: © 2023 John Wiley & Sons A/S.. Published by John Wiley & Sons Ltd. Kévin Serror and Lauren Ferrero cofirst authors who equally contributed to this work.. 1INSERM UMR_S 976, Skin Research Center, SaintLouis Hospital, Paris, France 2Paris University, Paris, France 3Department of Reconstructive and Plastic Surgery, SaintLouis Hospital, Paris, France 4INSERM UMR_S 976, CEA CytoMorphoLab, SaintLouis Hospital, Paris, France 5Genomics Platform, Translational Research Department, Research Center, Institut Curie, Paris Sciences et Lettres (PSL) Research University, Paris, France 6GenoSplice, Paris Biotech Santé, Paris, France 7Dermatology Department, SaintLouis Hospital, Paris, France

Article Title: Reply to: High-Dose Inhaled Nitric Oxide in Acute Hypoxemic Respiratory Failure: Need for Patient Phenotyping?
Article Snippet: Rationale: Within chronic obstructive pulmonary disease (COPD), emphysema is characterized by a significant yet partially understood B cell immune component.. Objective: To characterize the transcriptomic signatures from lymphoid follicles (LFs) in ever-smokers without COPD and COPD patients with varying degrees of emphysema.. Methods: Lung sections from 40 COPD patients and ever-smokers were used for LF proteomic and transcriptomic spatial profiling.

Article Title: Duox is the primary NADPH oxidase responsible for ROS production during adult caudal fin regeneration in zebrafish
Article Snippet: MetaMorph® software was used to setup the mircroscope to capture two wavelengths - HyPer low (CFP) emission 482/25 and HyPer high (YFP) emission 544/24.

Article Title: Antibodies and antigen-binding fragments that specifically bind to microtubule-associated protein tau
Article Snippet: Representative images were acquired with Olympus BX-41 upright microscope using METAMORPH® software.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase
Article Snippet: Metamorph® Software was used to quantify Tryptase + and TLR7 + cells.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase.
Article Snippet: Metamorph® Software was used to quantify Tryptase+ and TLR7+ cells.

Article Title: Heteromultivalent particle compositions
Article Snippet: Data Analysis For in vitro and ex vivo fluorescence images of liposome-incubated platelets, surface averaged fluorescence intensity was measured using Metamorph® software.

Article Title: NLRP12 is an innate immune checkpoint for repressing IFN signatures and attenuating lupus nephritis progression
Article Snippet: Flowchart of calculating PAS staining area of kidney section by metamorph® software.

Saline:

Article Title: Evidence of inter- and intra-keloid heterogeneity through analysis of dermal fibroblasts: A new insight in deciphering keloid physiopathology.
Article Snippet: © 2023 John Wiley & Sons A/S.. Published by John Wiley & Sons Ltd. Kévin Serror and Lauren Ferrero cofirst authors who equally contributed to this work.. 1INSERM UMR_S 976, Skin Research Center, SaintLouis Hospital, Paris, France 2Paris University, Paris, France 3Department of Reconstructive and Plastic Surgery, SaintLouis Hospital, Paris, France 4INSERM UMR_S 976, CEA CytoMorphoLab, SaintLouis Hospital, Paris, France 5Genomics Platform, Translational Research Department, Research Center, Institut Curie, Paris Sciences et Lettres (PSL) Research University, Paris, France 6GenoSplice, Paris Biotech Santé, Paris, France 7Dermatology Department, SaintLouis Hospital, Paris, France

Article Title: Reply to: High-Dose Inhaled Nitric Oxide in Acute Hypoxemic Respiratory Failure: Need for Patient Phenotyping?
Article Snippet: Rationale: Within chronic obstructive pulmonary disease (COPD), emphysema is characterized by a significant yet partially understood B cell immune component.. Objective: To characterize the transcriptomic signatures from lymphoid follicles (LFs) in ever-smokers without COPD and COPD patients with varying degrees of emphysema.. Methods: Lung sections from 40 COPD patients and ever-smokers were used for LF proteomic and transcriptomic spatial profiling.

Article Title: Duox is the primary NADPH oxidase responsible for ROS production during adult caudal fin regeneration in zebrafish
Article Snippet: MetaMorph® software was used to setup the mircroscope to capture two wavelengths - HyPer low (CFP) emission 482/25 and HyPer high (YFP) emission 544/24.

Article Title: Antibodies and antigen-binding fragments that specifically bind to microtubule-associated protein tau
Article Snippet: Representative images were acquired with Olympus BX-41 upright microscope using METAMORPH® software.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase
Article Snippet: Metamorph® Software was used to quantify Tryptase + and TLR7 + cells.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase.
Article Snippet: Metamorph® Software was used to quantify Tryptase+ and TLR7+ cells.

Article Title: Heteromultivalent particle compositions
Article Snippet: Data Analysis For in vitro and ex vivo fluorescence images of liposome-incubated platelets, surface averaged fluorescence intensity was measured using Metamorph® software.

Article Title: NLRP12 is an innate immune checkpoint for repressing IFN signatures and attenuating lupus nephritis progression
Article Snippet: Flowchart of calculating PAS staining area of kidney section by metamorph® software.

Expressing:

Article Title: Evidence of inter- and intra-keloid heterogeneity through analysis of dermal fibroblasts: A new insight in deciphering keloid physiopathology.
Article Snippet: © 2023 John Wiley & Sons A/S.. Published by John Wiley & Sons Ltd. Kévin Serror and Lauren Ferrero cofirst authors who equally contributed to this work.. 1INSERM UMR_S 976, Skin Research Center, SaintLouis Hospital, Paris, France 2Paris University, Paris, France 3Department of Reconstructive and Plastic Surgery, SaintLouis Hospital, Paris, France 4INSERM UMR_S 976, CEA CytoMorphoLab, SaintLouis Hospital, Paris, France 5Genomics Platform, Translational Research Department, Research Center, Institut Curie, Paris Sciences et Lettres (PSL) Research University, Paris, France 6GenoSplice, Paris Biotech Santé, Paris, France 7Dermatology Department, SaintLouis Hospital, Paris, France

Article Title: Reply to: High-Dose Inhaled Nitric Oxide in Acute Hypoxemic Respiratory Failure: Need for Patient Phenotyping?
Article Snippet: Rationale: Within chronic obstructive pulmonary disease (COPD), emphysema is characterized by a significant yet partially understood B cell immune component.. Objective: To characterize the transcriptomic signatures from lymphoid follicles (LFs) in ever-smokers without COPD and COPD patients with varying degrees of emphysema.. Methods: Lung sections from 40 COPD patients and ever-smokers were used for LF proteomic and transcriptomic spatial profiling.

Article Title: Duox is the primary NADPH oxidase responsible for ROS production during adult caudal fin regeneration in zebrafish
Article Snippet: MetaMorph® software was used to setup the mircroscope to capture two wavelengths - HyPer low (CFP) emission 482/25 and HyPer high (YFP) emission 544/24.

Article Title: Antibodies and antigen-binding fragments that specifically bind to microtubule-associated protein tau
Article Snippet: Representative images were acquired with Olympus BX-41 upright microscope using METAMORPH® software.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase
Article Snippet: Metamorph® Software was used to quantify Tryptase + and TLR7 + cells.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase.
Article Snippet: Metamorph® Software was used to quantify Tryptase+ and TLR7+ cells.

Article Title: Heteromultivalent particle compositions
Article Snippet: Data Analysis For in vitro and ex vivo fluorescence images of liposome-incubated platelets, surface averaged fluorescence intensity was measured using Metamorph® software.

Article Title: NLRP12 is an innate immune checkpoint for repressing IFN signatures and attenuating lupus nephritis progression
Article Snippet: Flowchart of calculating PAS staining area of kidney section by metamorph® software.

Derivative Assay:

Article Title: Evidence of inter- and intra-keloid heterogeneity through analysis of dermal fibroblasts: A new insight in deciphering keloid physiopathology.
Article Snippet: © 2023 John Wiley & Sons A/S.. Published by John Wiley & Sons Ltd. Kévin Serror and Lauren Ferrero cofirst authors who equally contributed to this work.. 1INSERM UMR_S 976, Skin Research Center, SaintLouis Hospital, Paris, France 2Paris University, Paris, France 3Department of Reconstructive and Plastic Surgery, SaintLouis Hospital, Paris, France 4INSERM UMR_S 976, CEA CytoMorphoLab, SaintLouis Hospital, Paris, France 5Genomics Platform, Translational Research Department, Research Center, Institut Curie, Paris Sciences et Lettres (PSL) Research University, Paris, France 6GenoSplice, Paris Biotech Santé, Paris, France 7Dermatology Department, SaintLouis Hospital, Paris, France

Article Title: Reply to: High-Dose Inhaled Nitric Oxide in Acute Hypoxemic Respiratory Failure: Need for Patient Phenotyping?
Article Snippet: Rationale: Within chronic obstructive pulmonary disease (COPD), emphysema is characterized by a significant yet partially understood B cell immune component.. Objective: To characterize the transcriptomic signatures from lymphoid follicles (LFs) in ever-smokers without COPD and COPD patients with varying degrees of emphysema.. Methods: Lung sections from 40 COPD patients and ever-smokers were used for LF proteomic and transcriptomic spatial profiling.

Article Title: Duox is the primary NADPH oxidase responsible for ROS production during adult caudal fin regeneration in zebrafish
Article Snippet: MetaMorph® software was used to setup the mircroscope to capture two wavelengths - HyPer low (CFP) emission 482/25 and HyPer high (YFP) emission 544/24.

Article Title: Antibodies and antigen-binding fragments that specifically bind to microtubule-associated protein tau
Article Snippet: Representative images were acquired with Olympus BX-41 upright microscope using METAMORPH® software.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase
Article Snippet: Metamorph® Software was used to quantify Tryptase + and TLR7 + cells.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase.
Article Snippet: Metamorph® Software was used to quantify Tryptase+ and TLR7+ cells.

Article Title: Heteromultivalent particle compositions
Article Snippet: Data Analysis For in vitro and ex vivo fluorescence images of liposome-incubated platelets, surface averaged fluorescence intensity was measured using Metamorph® software.

Article Title: NLRP12 is an innate immune checkpoint for repressing IFN signatures and attenuating lupus nephritis progression
Article Snippet: Flowchart of calculating PAS staining area of kidney section by metamorph® software.

Incubation:

Article Title: Evidence of inter- and intra-keloid heterogeneity through analysis of dermal fibroblasts: A new insight in deciphering keloid physiopathology.
Article Snippet: © 2023 John Wiley & Sons A/S.. Published by John Wiley & Sons Ltd. Kévin Serror and Lauren Ferrero cofirst authors who equally contributed to this work.. 1INSERM UMR_S 976, Skin Research Center, SaintLouis Hospital, Paris, France 2Paris University, Paris, France 3Department of Reconstructive and Plastic Surgery, SaintLouis Hospital, Paris, France 4INSERM UMR_S 976, CEA CytoMorphoLab, SaintLouis Hospital, Paris, France 5Genomics Platform, Translational Research Department, Research Center, Institut Curie, Paris Sciences et Lettres (PSL) Research University, Paris, France 6GenoSplice, Paris Biotech Santé, Paris, France 7Dermatology Department, SaintLouis Hospital, Paris, France

Article Title: Reply to: High-Dose Inhaled Nitric Oxide in Acute Hypoxemic Respiratory Failure: Need for Patient Phenotyping?
Article Snippet: Rationale: Within chronic obstructive pulmonary disease (COPD), emphysema is characterized by a significant yet partially understood B cell immune component.. Objective: To characterize the transcriptomic signatures from lymphoid follicles (LFs) in ever-smokers without COPD and COPD patients with varying degrees of emphysema.. Methods: Lung sections from 40 COPD patients and ever-smokers were used for LF proteomic and transcriptomic spatial profiling.

Article Title: Duox is the primary NADPH oxidase responsible for ROS production during adult caudal fin regeneration in zebrafish
Article Snippet: MetaMorph® software was used to setup the mircroscope to capture two wavelengths - HyPer low (CFP) emission 482/25 and HyPer high (YFP) emission 544/24.

Article Title: Antibodies and antigen-binding fragments that specifically bind to microtubule-associated protein tau
Article Snippet: Representative images were acquired with Olympus BX-41 upright microscope using METAMORPH® software.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase
Article Snippet: Metamorph® Software was used to quantify Tryptase + and TLR7 + cells.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase.
Article Snippet: Metamorph® Software was used to quantify Tryptase+ and TLR7+ cells.

Article Title: Heteromultivalent particle compositions
Article Snippet: Data Analysis For in vitro and ex vivo fluorescence images of liposome-incubated platelets, surface averaged fluorescence intensity was measured using Metamorph® software.

Article Title: NLRP12 is an innate immune checkpoint for repressing IFN signatures and attenuating lupus nephritis progression
Article Snippet: Flowchart of calculating PAS staining area of kidney section by metamorph® software.

Activity Assay:

Article Title: Evidence of inter- and intra-keloid heterogeneity through analysis of dermal fibroblasts: A new insight in deciphering keloid physiopathology.
Article Snippet: © 2023 John Wiley & Sons A/S.. Published by John Wiley & Sons Ltd. Kévin Serror and Lauren Ferrero cofirst authors who equally contributed to this work.. 1INSERM UMR_S 976, Skin Research Center, SaintLouis Hospital, Paris, France 2Paris University, Paris, France 3Department of Reconstructive and Plastic Surgery, SaintLouis Hospital, Paris, France 4INSERM UMR_S 976, CEA CytoMorphoLab, SaintLouis Hospital, Paris, France 5Genomics Platform, Translational Research Department, Research Center, Institut Curie, Paris Sciences et Lettres (PSL) Research University, Paris, France 6GenoSplice, Paris Biotech Santé, Paris, France 7Dermatology Department, SaintLouis Hospital, Paris, France

Article Title: Reply to: High-Dose Inhaled Nitric Oxide in Acute Hypoxemic Respiratory Failure: Need for Patient Phenotyping?
Article Snippet: Rationale: Within chronic obstructive pulmonary disease (COPD), emphysema is characterized by a significant yet partially understood B cell immune component.. Objective: To characterize the transcriptomic signatures from lymphoid follicles (LFs) in ever-smokers without COPD and COPD patients with varying degrees of emphysema.. Methods: Lung sections from 40 COPD patients and ever-smokers were used for LF proteomic and transcriptomic spatial profiling.

Article Title: Duox is the primary NADPH oxidase responsible for ROS production during adult caudal fin regeneration in zebrafish
Article Snippet: MetaMorph® software was used to setup the mircroscope to capture two wavelengths - HyPer low (CFP) emission 482/25 and HyPer high (YFP) emission 544/24.

Article Title: Antibodies and antigen-binding fragments that specifically bind to microtubule-associated protein tau
Article Snippet: Representative images were acquired with Olympus BX-41 upright microscope using METAMORPH® software.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase
Article Snippet: Metamorph® Software was used to quantify Tryptase + and TLR7 + cells.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase.
Article Snippet: Metamorph® Software was used to quantify Tryptase+ and TLR7+ cells.

Article Title: Heteromultivalent particle compositions
Article Snippet: Data Analysis For in vitro and ex vivo fluorescence images of liposome-incubated platelets, surface averaged fluorescence intensity was measured using Metamorph® software.

Article Title: NLRP12 is an innate immune checkpoint for repressing IFN signatures and attenuating lupus nephritis progression
Article Snippet: Flowchart of calculating PAS staining area of kidney section by metamorph® software.

In Vitro:

Article Title: Evidence of inter- and intra-keloid heterogeneity through analysis of dermal fibroblasts: A new insight in deciphering keloid physiopathology.
Article Snippet: © 2023 John Wiley & Sons A/S.. Published by John Wiley & Sons Ltd. Kévin Serror and Lauren Ferrero cofirst authors who equally contributed to this work.. 1INSERM UMR_S 976, Skin Research Center, SaintLouis Hospital, Paris, France 2Paris University, Paris, France 3Department of Reconstructive and Plastic Surgery, SaintLouis Hospital, Paris, France 4INSERM UMR_S 976, CEA CytoMorphoLab, SaintLouis Hospital, Paris, France 5Genomics Platform, Translational Research Department, Research Center, Institut Curie, Paris Sciences et Lettres (PSL) Research University, Paris, France 6GenoSplice, Paris Biotech Santé, Paris, France 7Dermatology Department, SaintLouis Hospital, Paris, France

Article Title: Reply to: High-Dose Inhaled Nitric Oxide in Acute Hypoxemic Respiratory Failure: Need for Patient Phenotyping?
Article Snippet: Rationale: Within chronic obstructive pulmonary disease (COPD), emphysema is characterized by a significant yet partially understood B cell immune component.. Objective: To characterize the transcriptomic signatures from lymphoid follicles (LFs) in ever-smokers without COPD and COPD patients with varying degrees of emphysema.. Methods: Lung sections from 40 COPD patients and ever-smokers were used for LF proteomic and transcriptomic spatial profiling.

Article Title: Duox is the primary NADPH oxidase responsible for ROS production during adult caudal fin regeneration in zebrafish
Article Snippet: MetaMorph® software was used to setup the mircroscope to capture two wavelengths - HyPer low (CFP) emission 482/25 and HyPer high (YFP) emission 544/24.

Article Title: Antibodies and antigen-binding fragments that specifically bind to microtubule-associated protein tau
Article Snippet: Representative images were acquired with Olympus BX-41 upright microscope using METAMORPH® software.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase
Article Snippet: Metamorph® Software was used to quantify Tryptase + and TLR7 + cells.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase.
Article Snippet: Metamorph® Software was used to quantify Tryptase+ and TLR7+ cells.

Article Title: Heteromultivalent particle compositions
Article Snippet: Data Analysis For in vitro and ex vivo fluorescence images of liposome-incubated platelets, surface averaged fluorescence intensity was measured using Metamorph® software.

Article Title: NLRP12 is an innate immune checkpoint for repressing IFN signatures and attenuating lupus nephritis progression
Article Snippet: Flowchart of calculating PAS staining area of kidney section by metamorph® software.

Ex Vivo:

Article Title: Evidence of inter- and intra-keloid heterogeneity through analysis of dermal fibroblasts: A new insight in deciphering keloid physiopathology.
Article Snippet: © 2023 John Wiley & Sons A/S.. Published by John Wiley & Sons Ltd. Kévin Serror and Lauren Ferrero cofirst authors who equally contributed to this work.. 1INSERM UMR_S 976, Skin Research Center, SaintLouis Hospital, Paris, France 2Paris University, Paris, France 3Department of Reconstructive and Plastic Surgery, SaintLouis Hospital, Paris, France 4INSERM UMR_S 976, CEA CytoMorphoLab, SaintLouis Hospital, Paris, France 5Genomics Platform, Translational Research Department, Research Center, Institut Curie, Paris Sciences et Lettres (PSL) Research University, Paris, France 6GenoSplice, Paris Biotech Santé, Paris, France 7Dermatology Department, SaintLouis Hospital, Paris, France

Article Title: Reply to: High-Dose Inhaled Nitric Oxide in Acute Hypoxemic Respiratory Failure: Need for Patient Phenotyping?
Article Snippet: Rationale: Within chronic obstructive pulmonary disease (COPD), emphysema is characterized by a significant yet partially understood B cell immune component.. Objective: To characterize the transcriptomic signatures from lymphoid follicles (LFs) in ever-smokers without COPD and COPD patients with varying degrees of emphysema.. Methods: Lung sections from 40 COPD patients and ever-smokers were used for LF proteomic and transcriptomic spatial profiling.

Article Title: Duox is the primary NADPH oxidase responsible for ROS production during adult caudal fin regeneration in zebrafish
Article Snippet: MetaMorph® software was used to setup the mircroscope to capture two wavelengths - HyPer low (CFP) emission 482/25 and HyPer high (YFP) emission 544/24.

Article Title: Antibodies and antigen-binding fragments that specifically bind to microtubule-associated protein tau
Article Snippet: Representative images were acquired with Olympus BX-41 upright microscope using METAMORPH® software.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase
Article Snippet: Metamorph® Software was used to quantify Tryptase + and TLR7 + cells.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase.
Article Snippet: Metamorph® Software was used to quantify Tryptase+ and TLR7+ cells.

Article Title: Heteromultivalent particle compositions
Article Snippet: Data Analysis For in vitro and ex vivo fluorescence images of liposome-incubated platelets, surface averaged fluorescence intensity was measured using Metamorph® software.

Article Title: NLRP12 is an innate immune checkpoint for repressing IFN signatures and attenuating lupus nephritis progression
Article Snippet: Flowchart of calculating PAS staining area of kidney section by metamorph® software.

Fluorescence:

Article Title: Evidence of inter- and intra-keloid heterogeneity through analysis of dermal fibroblasts: A new insight in deciphering keloid physiopathology.
Article Snippet: © 2023 John Wiley & Sons A/S.. Published by John Wiley & Sons Ltd. Kévin Serror and Lauren Ferrero cofirst authors who equally contributed to this work.. 1INSERM UMR_S 976, Skin Research Center, SaintLouis Hospital, Paris, France 2Paris University, Paris, France 3Department of Reconstructive and Plastic Surgery, SaintLouis Hospital, Paris, France 4INSERM UMR_S 976, CEA CytoMorphoLab, SaintLouis Hospital, Paris, France 5Genomics Platform, Translational Research Department, Research Center, Institut Curie, Paris Sciences et Lettres (PSL) Research University, Paris, France 6GenoSplice, Paris Biotech Santé, Paris, France 7Dermatology Department, SaintLouis Hospital, Paris, France

Article Title: Reply to: High-Dose Inhaled Nitric Oxide in Acute Hypoxemic Respiratory Failure: Need for Patient Phenotyping?
Article Snippet: Rationale: Within chronic obstructive pulmonary disease (COPD), emphysema is characterized by a significant yet partially understood B cell immune component.. Objective: To characterize the transcriptomic signatures from lymphoid follicles (LFs) in ever-smokers without COPD and COPD patients with varying degrees of emphysema.. Methods: Lung sections from 40 COPD patients and ever-smokers were used for LF proteomic and transcriptomic spatial profiling.

Article Title: Duox is the primary NADPH oxidase responsible for ROS production during adult caudal fin regeneration in zebrafish
Article Snippet: MetaMorph® software was used to setup the mircroscope to capture two wavelengths - HyPer low (CFP) emission 482/25 and HyPer high (YFP) emission 544/24.

Article Title: Antibodies and antigen-binding fragments that specifically bind to microtubule-associated protein tau
Article Snippet: Representative images were acquired with Olympus BX-41 upright microscope using METAMORPH® software.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase
Article Snippet: Metamorph® Software was used to quantify Tryptase + and TLR7 + cells.

Article Title: TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase.
Article Snippet: Metamorph® Software was used to quantify Tryptase+ and TLR7+ cells.

Article Title: Heteromultivalent particle compositions
Article Snippet: Data Analysis For in vitro and ex vivo fluorescence images of liposome-incubated platelets, surface averaged fluorescence intensity was measured using Metamorph® software.

Article Title: NLRP12 is an innate immune checkpoint for repressing IFN signatures and attenuating lupus nephritis progression
Article Snippet: Flowchart of calculating PAS staining area of kidney section by metamorph® software.



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Ablating Pcbp1 in β cells upregulates PCBP2 and does not alter glucose homeostasis. (A) Model of Cre-mediated recombination of Pcbp1 . The top diagram represents the Pcbp1 locus (light boxes represent UTRs, dark box represents protein coding region, and arrow represents transcriptional start site) with inserted loxP sites (triangles). Bottom diagram represents the Pcbp1 locus following RIP-Cre mediated recombination and remaining loxP site (triangle). (B) Western blot showing PCBP1 levels in islets from 26 week old male Pcbp1 βKO mice ( Pcbp1 Fl/Fl ,n = 3; Pcbp1 βKO ,n = 3). (C) <t>Co-immunofluorescence</t> staining for PCBP1 and Insulin in adult islets from 7 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice (scale bar, 50 μm). (D) Intraperitoneal glucose tolerance tests performed on 8–11 week old male mice (n = 9 Pcbp1 Fl/Fl and n = 11 Pcbp1 βKO ). (E) Western blots showing PCBP2 levels in islets from 26 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice ( Pcbp1 Fl/Fl , n = 3; Pcbp1 βKO , n = 3). ∗P-value<0.05, ∗∗P-value<0.01 by student's t-test.
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Ablating Pcbp1 in β cells upregulates PCBP2 and does not alter glucose homeostasis. (A) Model of Cre-mediated recombination of Pcbp1 . The top diagram represents the Pcbp1 locus (light boxes represent UTRs, dark box represents protein coding region, and arrow represents transcriptional start site) with inserted loxP sites (triangles). Bottom diagram represents the Pcbp1 locus following RIP-Cre mediated recombination and remaining loxP site (triangle). (B) Western blot showing PCBP1 levels in islets from 26 week old male Pcbp1 βKO mice ( Pcbp1 Fl/Fl ,n = 3; Pcbp1 βKO ,n = 3). (C) Co-immunofluorescence staining for PCBP1 and Insulin in adult islets from 7 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice (scale bar, 50 μm). (D) Intraperitoneal glucose tolerance tests performed on 8–11 week old male mice (n = 9 Pcbp1 Fl/Fl and n = 11 Pcbp1 βKO ). (E) Western blots showing PCBP2 levels in islets from 26 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice ( Pcbp1 Fl/Fl , n = 3; Pcbp1 βKO , n = 3). ∗P-value<0.05, ∗∗P-value<0.01 by student's t-test.

Journal: Molecular Metabolism

Article Title: RNA binding proteins PCBP1 and PCBP2 regulate pancreatic β cell translation

doi: 10.1016/j.molmet.2025.102175

Figure Lengend Snippet: Ablating Pcbp1 in β cells upregulates PCBP2 and does not alter glucose homeostasis. (A) Model of Cre-mediated recombination of Pcbp1 . The top diagram represents the Pcbp1 locus (light boxes represent UTRs, dark box represents protein coding region, and arrow represents transcriptional start site) with inserted loxP sites (triangles). Bottom diagram represents the Pcbp1 locus following RIP-Cre mediated recombination and remaining loxP site (triangle). (B) Western blot showing PCBP1 levels in islets from 26 week old male Pcbp1 βKO mice ( Pcbp1 Fl/Fl ,n = 3; Pcbp1 βKO ,n = 3). (C) Co-immunofluorescence staining for PCBP1 and Insulin in adult islets from 7 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice (scale bar, 50 μm). (D) Intraperitoneal glucose tolerance tests performed on 8–11 week old male mice (n = 9 Pcbp1 Fl/Fl and n = 11 Pcbp1 βKO ). (E) Western blots showing PCBP2 levels in islets from 26 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice ( Pcbp1 Fl/Fl , n = 3; Pcbp1 βKO , n = 3). ∗P-value<0.05, ∗∗P-value<0.01 by student's t-test.

Article Snippet: Immunofluorescence images were visualized and captured using a Nikon Eclipse E600 microscope equipped with a QImaging Q/Click digital camera and Metamorph software.

Techniques: Western Blot, Immunofluorescence, Staining

Single cell RNA sequencing of Pcbp1 and Pcbp2 co-depleted β cells reveals dysregulation of a translational mRNA program. (A) Co-immunofluorescence staining for PCBP1 and PCBP2 in adult islets from 11–12 week old male Pcbp1/2 Fl/Fl and Pcbp1/2 βKO mice (scale bar, 50 μm). Yellow arrows denote PCBP1 and PCBP2 co-depleted β cells. (B) Merged uniform manifold approximation and projection (UMAP) plot showing 3,592 control β cells (red) and 1,554 Pcbp1/2 co-depleted β cells (blue). (C) UMAP plot showing identified β cell subclusters in control and mutant β cells. (D) Dotplot showing the relative expression of Ins1 , Pcbp1 , and Pcbp2 transcript levels in each identified β cell subcluster. (E) Volcano plot of genes with significantly downregulated (blue) and upregulated (red) transcript levels in cluster 11 as compared to control β cells. (F) Reactome pathway analysis of the genes with altered mRNA levels in the setting of Pcbp1/2 co-deficiency.

Journal: Molecular Metabolism

Article Title: RNA binding proteins PCBP1 and PCBP2 regulate pancreatic β cell translation

doi: 10.1016/j.molmet.2025.102175

Figure Lengend Snippet: Single cell RNA sequencing of Pcbp1 and Pcbp2 co-depleted β cells reveals dysregulation of a translational mRNA program. (A) Co-immunofluorescence staining for PCBP1 and PCBP2 in adult islets from 11–12 week old male Pcbp1/2 Fl/Fl and Pcbp1/2 βKO mice (scale bar, 50 μm). Yellow arrows denote PCBP1 and PCBP2 co-depleted β cells. (B) Merged uniform manifold approximation and projection (UMAP) plot showing 3,592 control β cells (red) and 1,554 Pcbp1/2 co-depleted β cells (blue). (C) UMAP plot showing identified β cell subclusters in control and mutant β cells. (D) Dotplot showing the relative expression of Ins1 , Pcbp1 , and Pcbp2 transcript levels in each identified β cell subcluster. (E) Volcano plot of genes with significantly downregulated (blue) and upregulated (red) transcript levels in cluster 11 as compared to control β cells. (F) Reactome pathway analysis of the genes with altered mRNA levels in the setting of Pcbp1/2 co-deficiency.

Article Snippet: Immunofluorescence images were visualized and captured using a Nikon Eclipse E600 microscope equipped with a QImaging Q/Click digital camera and Metamorph software.

Techniques: RNA Sequencing, Immunofluorescence, Staining, Control, Mutagenesis, Expressing